Hotspot

psaJ-rpl33

LSC · 66618-67041 · 424 bp

Back to Matthiola

Pi 0.0536

MarkerSeek score 81.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 424
variable_sites: 45
pi: 0.0536
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACCCGTGACATTTTGTACCC	TCGTTTCGAACACAACTGGT	50.0	45.0	58.1	58.0	998-1099 bp	1.0	90.1
psaJ-rpl33_p2	CCCGTGACATTTTGTACCCA	TCGTTTCGAACACAACTGGT	50.0	45.0	58.1	58.0	997-1098 bp	1.0	90.1
psaJ-rpl33_p3	ACAGGATTTGAACCCGTGAC	TCGTTTCGAACACAACTGGT	50.0	45.0	58.1	58.0	1009-1110 bp	1.0	89.9
psaJ-rpl33_p4	CAGGATTTGAACCCGTGACA	TCGTTTCGAACACAACTGGT	50.0	45.0	58.1	58.0	1008-1109 bp	1.0	89.9
psaJ-rpl33_p5	TGACAGGATTTGAACCCGTG	TCGTTTCGAACACAACTGGT	50.0	45.0	58.1	58.0	1011-1112 bp	0.8	81.5