Hotspot

trnP-UGG-psaJ

LSC · 66109-66488 · 380 bp

Back to Matthiola

Pi 0.0578

MarkerSeek score 81.80

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 380
variable_sites: 42
pi: 0.0578
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	GGAAAGATATGTTTTTAGATCTCGCA	45.0	34.6	57.9	57.9	419-465 bp	1.0	51.7
trnP-UGG-psaJ_p2	CAAAACAAACGCGCTACCAA	GGAAAGATATGTTTTTAGATCTCGCA	45.0	34.6	58.2	57.9	420-466 bp	1.0	51.6
trnP-UGG-psaJ_p3	AAAACAAACGCGCTACCAAG	ACGGAAAGATATGTTTTTAGATCTCG	45.0	34.6	57.9	57.6	421-467 bp	1.0	51.6
trnP-UGG-psaJ_p4	CAAAACAAACGCGCTACCAA	ACGGAAAGATATGTTTTTAGATCTCG	45.0	34.6	58.2	57.6	422-468 bp	1.0	51.6
trnP-UGG-psaJ_p5	TTGTACCCAAAACAAACGCG	GGAAAGATATGTTTTTAGATCTCGCA	45.0	34.6	57.8	57.9	427-473 bp	1.0	51.5