Hotspot

accD-psaI

LSC · 57949-58679 · 731 bp

Back to Matthiola

Pi 0.0504

MarkerSeek score 81.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 731
variable_sites: 68
pi: 0.0504
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGCCGAACCCTATGCCTATA	TCACAATCCCTTGCGGAAAA	50.0	45.0	58.0	58.0	1406-1795 bp	1.0	89.2
accD-psaI_p2	GGTAGCCATTGGGGTTATGG	TCACAATCCCTTGCGGAAAA	55.0	45.0	58.0	58.0	1706-2095 bp	1.0	88.6
accD-psaI_p3	TGACTGACGCTGTTCAAACA	TCACAATCCCTTGCGGAAAA	45.0	45.0	58.0	58.0	1752-2141 bp	1.0	88.5
accD-psaI_p4	ATGACAGGATTGACTGACGC	TCACAATCCCTTGCGGAAAA	50.0	45.0	58.0	58.0	1762-2151 bp	1.0	88.4
accD-psaI_p5	CATTCGCGGGAGGAAACTTA	TCACAATCCCTTGCGGAAAA	50.0	45.0	58.0	58.0	1810-2199 bp	1.0	88.4