Hotspot

rbcL-accD

LSC · 55775-56493 · 719 bp

Back to Matthiola

Pi 0.0498

MarkerSeek score 83.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 67
pi: 0.0498
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TGCAATGCACGCTGTTATTG	ATTGCTTTACCTAGCTCGCC	45.0	50.0	58.0	58.0	1311-1376 bp	1.0	88.1
rbcL-accD_p2	TTGAATGCTACTGCGGGTAC	ATTGCTTTACCTAGCTCGCC	50.0	50.0	58.0	58.0	1478-1543 bp	1.0	87.2
rbcL-accD_p3	TGCAATGCACGCTGTTATTG	ATTCCGAATGTCCTTGGAGC	45.0	50.0	58.0	58.0	1456-1500 bp	0.8	79.7
rbcL-accD_p4	TCATGGTATGCACTTCCGTG	ATTCCGAATGTCCTTGGAGC	50.0	50.0	58.0	58.0	1423-1467 bp	0.8	79.7
rbcL-accD_p5	TTGAATGCTACTGCGGGTAC	ATTCCGAATGTCCTTGGAGC	50.0	50.0	58.0	58.0	1623-1667 bp	0.8	79.4