Hotspot

trnE-UUC-trnT-GGU

LSC · 30247-30964 · 718 bp

Back to Matthiola

Pi 0.0527

MarkerSeek score 87.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 718
variable_sites: 71
pi: 0.0527
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TAACCACTAGACGATGGGGG	GAACCGATGACTTACGCCTT	55.0	50.0	58.2	58.0	515-913 bp	1.0	89.3
trnE-UUC-trnT-GGU_p2	TAACCACTAGACGATGGGGG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.2	58.1	494-892 bp	1.0	89.0
trnE-UUC-trnT-GGU_p3	TAACCACTAGACGATGGGGG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.2	58.1	491-889 bp	1.0	88.9
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	541-939 bp	1.0	88.7
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	520-918 bp	1.0	88.4