Hotspot

psbM-trnD-GUC

LSC · 28580-29517 · 938 bp

Back to Matthiola

Pi 0.0456

MarkerSeek score 80.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 938
variable_sites: 79
pi: 0.0456
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGCAAGAATATTGACTTCCA	TTCAATTGGTCAGAGCACCG	35.0	50.0	52.5	58.5	656-1113 bp	1.0	50.4
psbM-trnD-GUC_p2	ATGCAAGAATATTGACTTCCA	TTCAATTGGTCAGAGCACCG	33.3	50.0	53.2	58.5	657-1114 bp	1.0	50.4
psbM-trnD-GUC_p3	TGCAAGAATATTGACTTCCAT	TTCAATTGGTCAGAGCACCG	33.3	50.0	53.2	58.5	656-1113 bp	1.0	50.4
psbM-trnD-GUC_p4	AATGCAAGAATATTGACTTCCA	TTCAATTGGTCAGAGCACCG	31.8	50.0	54.0	58.5	658-1115 bp	1.0	50.4
psbM-trnD-GUC_p5	TGCAAGAATATTGACTTCCA	GTTCAATTGGTCAGAGCACC	35.0	50.0	52.5	57.0	657-1114 bp	1.0	50.4