Hotspot

trnG-GGA-trnR-UCU

LSC · 9218-9385 · 168 bp

Back to Matthiola

Pi 0.0879
MarkerSeek score 82.20
Primer available yes
Label trnG-GGA-trnR-UCU

Feature Metadata

label_name
trnG-GGA-trnR-UCU
feature_type
igs
region
LSC
length_bp
168
variable_sites
25
pi
0.0879
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GGA-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTATGTCCT 50.0 40.9 58.0 55.6 248-265 bp 1.0 55.7
trnG-GGA-trnR-UCU_p2 AGCCTTCCAAGCTAACGATG AGAAGACCTATGTCCTATCCA 50.0 42.9 58.0 54.6 242-259 bp 1.0 55.7
trnG-GGA-trnR-UCU_p3 ACCCTTAGCCTTCCAAGCTA AGGTTTAGAAGACCTATGTCCT 50.0 40.9 58.0 55.6 254-271 bp 1.0 55.5
trnG-GGA-trnR-UCU_p4 ACCCTTAGCCTTCCAAGCTA AGAAGACCTATGTCCTATCCA 50.0 42.9 58.0 54.6 248-265 bp 1.0 55.5
trnG-GGA-trnR-UCU_p5 AAACCCTTAGCCTTCCAAGC AGGTTTAGAAGACCTATGTCCT 50.0 40.9 58.1 55.6 256-273 bp 1.0 55.0