Hotspot

psbK-psbI

LSC · 6976-7474 · 499 bp

Back to Matthiola

Pi 0.0554

MarkerSeek score 81.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 499
variable_sites: 43
pi: 0.0554
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCAGCTGTAAG	ACGTCCTGGATCATTAGATAGGA	50.0	43.5	57.9	58.0	519-619 bp	1.0	65.7
psbK-psbI_p2	TGGCAAGCAGCTGTAAGTTT	ACGTCCTGGATCATTAGATAGGA	45.0	43.5	58.3	58.0	516-616 bp	1.0	63.8
psbK-psbI_p3	TTGGCAAGCAGCTGTAAGTT	ACGTCCTGGATCATTAGATAGGA	45.0	43.5	58.3	58.0	517-617 bp	1.0	63.7
psbK-psbI_p4	TTTGGCAAGCAGCTGTAAGT	ACGTCCTGGATCATTAGATAGGA	45.0	43.5	58.3	58.0	518-618 bp	1.0	63.7
psbK-psbI_p5	GTTTGGCAAGCAGCTGTAAG	GGATTACGTCCTGGATCATT	50.0	45.0	57.9	54.6	524-624 bp	1.0	62.6