Hotspot

trnK-UUU-rps16

LSC · 4338-4924 · 587 bp

Back to Matthiola

Pi 0.067
MarkerSeek score 84.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
587
variable_sites
81
pi
0.067
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG CATGCGTGAATCAAGCGTTT 55.0 45.0 58.0 58.0 1444-1512 bp 1.0 88.9
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 949-980 bp 0.8 79.9
trnK-UUU-rps16_p3 TGCGATACGGTCAAAGTGAG CATGCGTGAATCAAGCGTTT 50.0 45.0 58.0 58.0 2030 bp 0.2 47.7
trnK-UUU-rps16_p4 TGCGATACGGTCAAAGTGAG CCCAATGAGCCGTTTATCGA 50.0 50.0 58.0 58.1 1535 bp 0.2 47.5
trnK-UUU-rps16_p5 TGCGATACGGTCAAAGTGAG TCCCAATGAGCCGTTTATCG 50.0 50.0 58.0 58.1 1536 bp 0.2 47.5