Hotspot

trnH-GUG-psbA

LSC · 75-371 · 297 bp

Back to Matthiola

Pi 0.11

MarkerSeek score 72.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 297
variable_sites: 61
pi: 0.11
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CCACTGCCTTATCCACTTGG	ATATTCAGCTCCCGTTGCAG	55.0	50.0	58.2	58.0	952 bp	0.2	45.7
trnH-GUG-psbA_p2	CCACTGCCTTATCCACTTGG	TGTTGAGGCTCCATCTACCA	55.0	50.0	58.2	58.0	355 bp	0.2	45.7
trnH-GUG-psbA_p3	CCACTGCCTTATCCACTTGG	GCTGTTGAGGCTCCATCTAC	55.0	55.0	58.2	58.1	357 bp	0.2	45.6
trnH-GUG-psbA_p4	CCACTGCCTTATCCACTTGG	ATGCACCCATTCCACATGTT	55.0	45.0	58.2	58.1	810 bp	0.2	45.6
trnH-GUG-psbA_p5	CCACTGCCTTATCCACTTGG	GGTGTAGCTGGTGTATTCGG	55.0	55.0	58.2	58.1	789 bp	0.2	45.5