Hotspot

ndhD

SSC · 113608-115110 · 1503 bp

Back to Matsumurella

Pi 0.00253

MarkerSeek score 45.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 8
pi: 0.00253
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CGGCCCTTTTATGTGGATCA	GATACCCTGGGACGGATGTA	50.0	55.0	57.9	58.0	2491-2499 bp	1.0	81.0
ndhD_p2	AGTTGGGACCCAAAGGAAAC	GATACCCTGGGACGGATGTA	50.0	55.0	57.9	58.0	2171-2179 bp	1.0	80.6
ndhD_p3	GTTGGGACCCAAAGGAAACT	GATACCCTGGGACGGATGTA	50.0	55.0	57.9	58.0	2170-2178 bp	1.0	80.6
ndhD_p4	GGCCCTTTGTCATTGTACCA	GATACCCTGGGACGGATGTA	50.0	55.0	58.1	58.0	1841-1847 bp	1.0	80.4
ndhD_p5	CTAACGAAGCATGGGGATCC	GATACCCTGGGACGGATGTA	55.0	55.0	58.1	58.0	2197-2205 bp	1.0	80.3