Hotspot

ndhD-psaC

SSC · 115111-115226 · 116 bp

Back to Matsumurella

Pi 0.0534

MarkerSeek score 70.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 116
variable_sites: 11
pi: 0.0534
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGCAACCCGCAAATATTGGT	ATGGCATGAAACAACTCGCA	45.0	45.0	58.4	58.8	213-214 bp	1.0	74.9
ndhD-psaC_p2	AAAAATGAAGCAACCCGCAA	ATGGCATGAAACAACTCGCA	40.0	45.0	57.4	58.8	221-222 bp	1.0	73.4
ndhD-psaC_p3	AAAATGAAGCAACCCGCAAA	ATGGCATGAAACAACTCGCA	40.0	45.0	57.4	58.8	220-221 bp	1.0	73.4
ndhD-psaC_p4	AAGCAACCCGCAAATATTGG	ATGGCATGAAACAACTCGCA	45.0	45.0	57.3	58.8	214-215 bp	1.0	73.1
ndhD-psaC_p5	AAATGAAGCAACCCGCAAAT	ATGGCATGAAACAACTCGCA	40.0	45.0	57.2	58.8	219-220 bp	1.0	71.6