Hotspot

rpl20-rps12

LSC · 68153-68920 · 768 bp

Back to Matsumurella

Pi 0.00548

MarkerSeek score 56.40

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 768
variable_sites: 8
pi: 0.00548
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TGAATGAGACCCCCGAAAAC	ACAGCCCATTCGAAATGTCA	50.0	45.0	57.8	57.8	932-934 bp	1.0	77.7
rpl20-rps12_p2	AGACCCCCGAAAACTTGATG	ACAGCCCATTCGAAATGTCA	50.0	45.0	57.8	57.8	926-928 bp	1.0	77.7
rpl20-rps12_p3	ATGAGACCCCCGAAAACTTG	ACAGCCCATTCGAAATGTCA	50.0	45.0	57.8	57.8	929-931 bp	1.0	77.7
rpl20-rps12_p4	TGAATGAGACCCCCGAAAAC	ACAAGACAGCCCATTCGAAA	50.0	45.0	57.8	57.7	937-939 bp	1.0	77.1
rpl20-rps12_p5	AGACCCCCGAAAACTTGATG	ACAAGACAGCCCATTCGAAA	50.0	45.0	57.8	57.7	931-933 bp	1.0	77.1