Hotspot

atpB-rbcL

LSC · 53807-54587 · 781 bp

Back to Matsumurella

Pi 0.00576

MarkerSeek score 55.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 9
pi: 0.00576
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GATGATACGCCCCTGGTTTT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.9	58.3	925-926 bp	1.0	77.9
atpB-rbcL_p2	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	892-893 bp	1.0	77.7
atpB-rbcL_p3	ATTTGGATGATACGCCCCTG	AACACCCGCTTTGAATCCAA	50.0	45.0	57.7	58.3	930-931 bp	1.0	76.1
atpB-rbcL_p4	GATGATACGCCCCTGGTTTT	TAACACCCGCTTTGAATCCA	50.0	45.0	57.9	57.4	926-927 bp	1.0	75.8
atpB-rbcL_p5	GGAAACCCCAGAACCAGAAG	TAACACCCGCTTTGAATCCA	55.0	45.0	58.1	57.4	893-894 bp	1.0	75.5