Hotspot

atpH-atpI

LSC · 13623-14621 · 999 bp

Back to Matsumurella

Pi 0.00991

MarkerSeek score 59.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 999
variable_sites: 18
pi: 0.00991
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.0	57.9	1126-1133 bp	1.0	80.5
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	CCCGTCATGTTCCTTGGATT	45.0	50.0	57.9	57.9	1142-1149 bp	1.0	79.6
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.2	57.9	1127-1134 bp	1.0	79.3
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.8	57.9	1141-1148 bp	1.0	79.1
atpH-atpI_p5	TCCAGCAGCAATAACGGAAG	CCCGTCATGTTCCTTGGATT	50.0	50.0	58.3	57.9	1136-1143 bp	1.0	78.7