Hotspot

ycf1

SSC · 125969-131440 · 5472 bp

Back to Masdevallia

Pi 0.0159

MarkerSeek score 54.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5472
variable_sites: 87
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCCTTGATGTTTAAGTACAGGT	TCCATTTACAACAATGAACCA	39.1	33.3	57.2	53.6	217 bp	1.0	42.6
ycf1_p2	TCCCTTGATGTTTAAGTACAGGT	TCCATTTACAACAATGAACCAA	39.1	31.8	57.2	54.4	217 bp	1.0	42.6
ycf1_p3	TGATGTTTAAGTACAGGTCTCCA	TCCATTTACAACAATGAACCA	39.1	33.3	56.9	53.6	212 bp	1.0	42.6
ycf1_p4	CCCTTGATGTTTAAGTACAGGTC	TCCATTTACAACAATGAACCA	43.5	33.3	56.9	53.6	216 bp	1.0	42.6
ycf1_p5	TCCCGTGTATCATAAAAGAGTCT	TCCATTTACAACAATGAACCA	39.1	33.3	56.8	53.6	268 bp	1.0	42.1