Hotspot

rpl32-trnL-UAG

SSC · 115173-115983 · 811 bp

Back to Masdevallia

Pi 0.038

MarkerSeek score 78.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 811
variable_sites: 29
pi: 0.038
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	869-910 bp	1.0	85.3
rpl32-trnL-UAG_p2	TCCACCGGACAGTCAAAAAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	862-903 bp	1.0	84.8
rpl32-trnL-UAG_p3	TCCACCGGACAGTCAAAAAG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	868-909 bp	1.0	84.7
rpl32-trnL-UAG_p4	TCCACCGGACAGTCAAAAAG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	867-908 bp	1.0	84.7
rpl32-trnL-UAG_p5	TCCACCGGACAGTCAAAAAG	GAGCAGCGTGTCTACCAATT	50.0	50.0	58.0	58.3	863-904 bp	1.0	84.1