Hotspot

ndhF-rpl32

SSC · 114156-114998 · 843 bp

Back to Masdevallia

Pi 0.0242

MarkerSeek score 76.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 843
variable_sites: 19
pi: 0.0242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCACATCCGACGAAGATCTT	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	57.4	57.3	998-1055 bp	1.0	50.8
ndhF-rpl32_p2	CCACATCCGACGAAGATCTT	AGAAAAAGCTTTTACTGCCTCT	50.0	36.4	57.4	56.2	1005-1062 bp	1.0	50.2
ndhF-rpl32_p3	ACTGGGAGTGGAAAAAGAGG	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	57.0	57.3	931-988 bp	1.0	48.0
ndhF-rpl32_p4	CTGGGAGTGGAAAAAGAGGT	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	57.0	57.3	930-987 bp	1.0	48.0
ndhF-rpl32_p5	CCACATCCGACGAAGATCTT	AAGAAAAAGCTTTTACTGCCTCT	50.0	34.8	57.4	56.9	1006-1063 bp	1.0	47.9