Hotspot

psaJ-rpl33

LSC · 66923-67511 · 589 bp

Back to Masdevallia

Pi 0.0204

MarkerSeek score 68.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 589
variable_sites: 12
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GATGCCTTGTCATTCCCCTT	GCCTGGCGATTCTTTATTGC	50.0	50.0	57.9	57.9	709-714 bp	1.0	79.4
psaJ-rpl33_p2	GATGCCTTGTCATTCCCCTT	AGAAATGCCTGGCGATTCTT	50.0	45.0	57.9	57.9	715-720 bp	1.0	79.3
psaJ-rpl33_p3	TGGTTTGGGTCTTTAGCAGG	GCCTGGCGATTCTTTATTGC	50.0	50.0	57.7	57.9	760-765 bp	1.0	78.2
psaJ-rpl33_p4	GGTTTGGGTCTTTAGCAGGT	GCCTGGCGATTCTTTATTGC	50.0	50.0	57.7	57.9	759-764 bp	1.0	78.2
psaJ-rpl33_p5	TGGTTTGGGTCTTTAGCAGG	AGAAATGCCTGGCGATTCTT	50.0	45.0	57.7	57.9	766-771 bp	1.0	78.1