Hotspot

rbcL-accD

LSC · 55898-56739 · 842 bp

Back to Masdevallia

Pi 0.0297
MarkerSeek score 63.60
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
842
variable_sites
25
pi
0.0297
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AGTGGATAAGCTAGATAAAGAGACA ACAACATCGAATTGAACCAACA 36.0 36.4 56.9 57.1 904-910 bp 1.0 42.3
rbcL-accD_p2 AGTGGATAAGCTAGATAAAGAGACA AGACAACATCGAATTGAACCAAC 36.0 39.1 56.9 57.9 906-912 bp 1.0 42.3
rbcL-accD_p3 AGTGGATAAGCTAGATAAAGAGACA GACAACATCGAATTGAACCAACA 36.0 39.1 56.9 58.1 905-911 bp 1.0 42.3
rbcL-accD_p4 AGTGGATAAGCTAGATAAAGAGACA AGACAACATCGAATTGAACCA 36.0 38.1 56.9 55.8 906-912 bp 1.0 42.3
rbcL-accD_p5 GTGGATAAGCTAGATAAAGAGACA ACAACATCGAATTGAACCAACA 37.5 36.4 55.5 57.1 903-909 bp 1.0 42.3