Hotspot

atpB-rbcL

LSC · 53332-54424 · 1093 bp

Back to Masdevallia

Pi 0.0397

MarkerSeek score 78.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1093
variable_sites: 41
pi: 0.0397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	1161-1209 bp	1.0	84.7
atpB-rbcL_p2	GCCCTAGGTTTTGCTCTTCA	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.8	58.1	1138-1186 bp	1.0	84.3
atpB-rbcL_p3	ATACGCCCTAGGTTTTGCTC	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.7	58.1	1142-1190 bp	1.0	83.2
atpB-rbcL_p4	GATACGCCCTAGGTTTTGCT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.7	58.1	1143-1191 bp	1.0	83.2
atpB-rbcL_p5	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	1162-1210 bp	1.0	82.3