Hotspot

ndhC-trnV-UAC

LSC · 49718-50336 · 619 bp

Back to Masdevallia

Pi 0.023
MarkerSeek score 61.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
619
variable_sites
14
pi
0.023
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCTGGACCTTCCCTAATCGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.2 57.8 768-870 bp 1.0 79.1
ndhC-trnV-UAC_p2 TCTGGACCTTCCCTAATCGG GAAGGTCTACGGTTCGAGTC 55.0 55.0 58.2 57.5 767-869 bp 1.0 76.3
ndhC-trnV-UAC_p3 TCTGGACCTTCCCTAATCGG GTTCGAGTCCGTATAGCCCT 55.0 55.0 58.2 58.7 756-858 bp 1.0 75.2
ndhC-trnV-UAC_p4 TCTGGACCTTCCCTAATCGG AAGGTCTACGGTTCGAGTCC 55.0 55.0 58.2 58.8 766-868 bp 1.0 74.0
ndhC-trnV-UAC_p5 TCTGGACCTTCCCTAATCGG GAGAAGGTCTACGGTTCGAG 55.0 55.0 58.2 57.2 769-871 bp 1.0 74.0