Hotspot

trnS-UGA-psbZ

LSC · 35184-35421 · 238 bp

Back to Masdevallia

Pi 0.027

MarkerSeek score 69.90

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 238
variable_sites: 6
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	ACAACGGGTACGCTAATCAG	55.0	50.0	58.0	57.7	320-336 bp	1.0	82.9
trnS-UGA-psbZ_p2	CAACCACTCAGCCATCTCTC	CAACGGGTACGCTAATCAGT	55.0	50.0	58.0	57.7	319-335 bp	1.0	82.9
trnS-UGA-psbZ_p3	GTTTTCAAGACCGGGGCTAT	ACAACGGGTACGCTAATCAG	50.0	50.0	57.9	57.7	340-356 bp	1.0	81.8
trnS-UGA-psbZ_p4	GTTTTCAAGACCGGGGCTAT	CAACGGGTACGCTAATCAGT	50.0	50.0	57.9	57.7	339-355 bp	1.0	81.8
trnS-UGA-psbZ_p5	AGCAAAAGGAGAGAGAGGGA	CAACGGGTACGCTAATCAGT	50.0	50.0	57.7	57.7	397-413 bp	1.0	79.4