Hotspot

trnE-UUC-trnT-GGU

LSC · 31085-31805 · 721 bp

Back to Masdevallia

Pi 0.0148

MarkerSeek score 71.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 10
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	791-825 bp	1.0	83.6
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	CACCGCTGAAAAACGAAGAC	50.0	50.0	57.8	58.0	851-885 bp	1.0	83.4
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	ACCGCTGAAAAACGAAGACT	50.0	45.0	57.8	58.1	850-884 bp	1.0	83.2
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	TCACCGCTGAAAAACGAAGA	50.0	45.0	57.8	58.1	852-886 bp	1.0	83.1
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	770-804 bp	1.0	83.1