Hotspot

rpoB-trnC-GCA

LSC · 26507-27828 · 1322 bp

Back to Masdevallia

Pi 0.0228

MarkerSeek score 70.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1322
variable_sites: 30
pi: 0.0228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1470-1494 bp	1.0	76.2
rpoB-trnC-GCA_p2	AATTCTTCCGTCAAGCCCTG	CGACACCCAGATTCGAACTG	50.0	55.0	58.2	58.6	1494-1518 bp	1.0	75.2
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	TTTGTTGATCAGGCGACACC	50.0	50.0	58.2	58.8	1507-1531 bp	1.0	74.3
rpoB-trnC-GCA_p4	AAATTCTTCCGTCAAGCCCT	TAAAGGATTTGCAGTCCCCC	45.0	50.0	57.4	57.5	1471-1495 bp	1.0	72.9
rpoB-trnC-GCA_p5	AATTCTTCCGTCAAGCCCTG	ATAAAGGATTTGCAGTCCCCC	50.0	47.6	58.2	58.0	1471-1495 bp	1.0	72.2