Hotspot

trnG-UCC-trnR-UCU

LSC · 9527-9640 · 114 bp

Back to Masdevallia

Pi 0.0495

MarkerSeek score 76.20

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 114
variable_sites: 5
pi: 0.0495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	194-211 bp	1.0	69.3
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	197-214 bp	1.0	69.1
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	200-217 bp	1.0	68.6
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	203-220 bp	1.0	68.3
trnG-UCC-trnR-UCU_p5	CCAACGTCGACTATAACCCC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.8	56.9	215-232 bp	1.0	67.7