Hotspot

ndhI

Genome · 118581-119150 · 570 bp

Back to Marsilea

Pi 0.497

MarkerSeek score 75.10

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: Genome
length_bp: 570
variable_sites: 190
pi: 0.497
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TCCTTGCCATCACACATAGC	TTATCTGGGTGGGTGGGATT	50.0	50.0	58.0	58.0	1072 bp	0.5	59.6
ndhI_p2	TCCTTGCCATCACACATAGC	TTTATCTGGGTGGGTGGGAT	50.0	50.0	58.0	58.0	1073 bp	0.5	59.6
ndhI_p3	TCCTTGCCATCACACATAGC	AAGTTTCTTTTGCCCGTTGC	50.0	45.0	58.0	58.1	855 bp	0.5	59.1
ndhI_p4	TGCCATCACACATAGCGAAA	TTATCTGGGTGGGTGGGATT	45.0	50.0	57.9	58.0	1068 bp	0.5	59.1
ndhI_p5	TGCCATCACACATAGCGAAA	TTTATCTGGGTGGGTGGGAT	45.0	50.0	57.9	58.0	1069 bp	0.5	59.1