Hotspot

ndhH

Genome · 121475-122659 · 1185 bp

Back to Marsilea

Pi 0.495

MarkerSeek score 79.90

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: Genome
length_bp: 1185
variable_sites: 479
pi: 0.495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	ATCGCACGTAGGGATACAGA	GCGTAGGTTTGAACACTTGC	50.0	50.0	58.0	58.0	2414 bp	0.5	59.6
ndhH_p2	TCCTGCCATTAGAAGACCCA	GCGTAGGTTTGAACACTTGC	50.0	50.0	58.0	58.0	2301 bp	0.5	59.5
ndhH_p3	ATTATTCCCAACGGACCTGC	GCGTAGGTTTGAACACTTGC	50.0	50.0	57.9	58.0	2072 bp	0.5	59.3
ndhH_p4	TTTGTAGGTTTCGGTGCCAA	GCGTAGGTTTGAACACTTGC	45.0	50.0	57.9	58.0	1875 bp	0.5	59.3
ndhH_p5	ATCGCACGTAGGGATACAGA	ATTGTCGTTCCGCCAGATAC	50.0	50.0	58.0	58.1	1840 bp	0.5	59.3