Hotspot

ndhF

Genome · 108628-110817 · 2190 bp

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Pi 0.497
MarkerSeek score 79.00
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
Genome
length_bp
2190
variable_sites
912
pi
0.497
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 AGGGAGAGATATGTGGACGG CTTCTTTCCAAAGCAGGGGT 55.0 50.0 58.0 58.0 2744 bp 0.5 60.0
ndhF_p2 GAGAGATATGTGGACGGGGA CTTCTTTCCAAAGCAGGGGT 55.0 50.0 58.0 58.0 2741 bp 0.5 60.0
ndhF_p3 AGGGAGAGATATGTGGACGG CGGATCCGTGACAAATGAGT 55.0 50.0 58.0 58.0 2667 bp 0.5 59.9
ndhF_p4 GAGAGATATGTGGACGGGGA CGGATCCGTGACAAATGAGT 55.0 50.0 58.0 58.0 2664 bp 0.5 59.9
ndhF_p5 AGGGAGAGATATGTGGACGG AATGTTAACGTCGGACCTCG 55.0 50.0 58.0 58.0 2624 bp 0.5 59.9