Hotspot

chlN

Genome · 128508-129875 · 1368 bp

Back to Marsilea

Pi 0.469

MarkerSeek score 82.00

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: Genome
length_bp: 1368
variable_sites: 574
pi: 0.469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	CGCAACCCTACCCAATCTAC	TAGGGAAAAGGCGCATACAC	55.0	50.0	58.1	58.0	2102 bp	0.5	59.3
chlN_p2	CGTTTGTAGAGCGGAAGTCA	TAGGGAAAAGGCGCATACAC	50.0	50.0	57.9	58.0	2014 bp	0.5	59.3
chlN_p3	TGAGAACCGTTTGTAGAGCG	TAGGGAAAAGGCGCATACAC	50.0	50.0	57.9	58.0	2021 bp	0.5	59.3
chlN_p4	GGTCCGTGGCTAATATCGAC	TAGGGAAAAGGCGCATACAC	55.0	50.0	57.9	58.0	2129 bp	0.5	59.2
chlN_p5	CGCAACCCTACCCAATCTAC	GGTTAGGGAAAAGGCGCATA	55.0	50.0	58.1	58.0	2105 bp	0.5	59.2