Hotspot

chlL

Genome · 129947-130828 · 882 bp

Back to Marsilea

Pi 0.477

MarkerSeek score 82.50

Primer available yes

Label chlL

Feature Metadata

label_name: chlL
feature_type: gene
region: Genome
length_bp: 882
variable_sites: 380
pi: 0.477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	GCTATCCCCTCCAGATCCAT	AGGGAGAGATATGTGGACGG	55.0	55.0	58.0	58.0	1377 bp	0.5	59.7
chlL_p2	GCTATCCCCTCCAGATCCAT	GAGAGATATGTGGACGGGGA	55.0	55.0	58.0	58.0	1374 bp	0.5	59.7
chlL_p3	CTCCAACTTGGGTGCTATCC	AGGGAGAGATATGTGGACGG	55.0	55.0	58.0	58.0	1390 bp	0.5	59.6
chlL_p4	CTCCAACTTGGGTGCTATCC	GAGAGATATGTGGACGGGGA	55.0	55.0	58.0	58.0	1387 bp	0.5	59.6
chlL_p5	GCTATCCCCTCCAGATCCAT	CAAGACGTTAAGCCTCCTCC	55.0	55.0	58.0	58.0	1701 bp	0.5	59.6