Hotspot

rpl23-trnI-CAU

Genome · 87464-87702 · 239 bp

Back to Marsilea

Pi 0.128
MarkerSeek score 81.20
Primer available yes
Label rpl23-trnI-CAU

Feature Metadata

label_name
rpl23-trnI-CAU
feature_type
igs
region
Genome
length_bp
239
variable_sites
29
pi
0.128
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI-CAU_p1 ACTTGATTCTTTAGTCCATCCA TGGCGAATTCACAGGTTCAA 36.4 45.0 54.8 58.0 284-297 bp 1.0 51.6
rpl23-trnI-CAU_p2 ACTTGATTCTTTAGTCCATCCA TTGGCGAATTCACAGGTTCA 36.4 45.0 54.8 58.0 285-298 bp 1.0 51.5
rpl23-trnI-CAU_p3 ACTTGATTCTTTAGTCCATCCA AACGGTTAAAGCACCCAACT 36.4 45.0 54.8 57.9 310-323 bp 1.0 50.6
rpl23-trnI-CAU_p4 ACTTGATTCTTTAGTCCATCCA TGAACGGTTAAAGCACCCAA 36.4 45.0 54.8 57.9 312-325 bp 1.0 50.5
rpl23-trnI-CAU_p5 ACTTGATTCTTTAGTCCATCCA CATCCATGGCTGAACGGTTA 36.4 50.0 54.8 58.0 322-335 bp 1.0 50.2