Hotspot

psbE-petL

Genome · 65766-66984 · 1219 bp

Back to Marsilea

Pi 0.0743

MarkerSeek score 82.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 1219
variable_sites: 90
pi: 0.0743
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	GTAATGCTGTGGATGACCCA	GCGGATGTCAAAAAGGCAAA	50.0	45.0	57.6	57.9	1337-1353 bp	1.0	81.3
psbE-petL_p2	GTTCTCCCGTATTCCCAGAC	GCGGATGTCAAAAAGGCAAA	55.0	45.0	57.4	57.9	1282-1298 bp	1.0	80.1
psbE-petL_p3	AGTAATGCTGTGGATGACCC	GCGGATGTCAAAAAGGCAAA	50.0	45.0	57.3	57.9	1338-1354 bp	1.0	78.9
psbE-petL_p4	AGGACGTTCTCCCGTATTCC	GCGGATGTCAAAAAGGCAAA	55.0	45.0	58.9	57.9	1287-1303 bp	1.0	77.8
psbE-petL_p5	TTCTCCCGTATTCCCAGACG	GCGGATGTCAAAAAGGCAAA	55.0	45.0	58.9	57.9	1281-1297 bp	1.0	77.8