Hotspot

ycf12-trnG-UCC

Genome · 11569-11707 · 139 bp

Back to Marsilea

Pi 0.131

MarkerSeek score 81.20

Primer available yes

Label ycf12-trnG-UCC

Feature Metadata

label_name: ycf12-trnG-UCC
feature_type: igs
region: Genome
length_bp: 139
variable_sites: 17
pi: 0.131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf12-trnG-UCC_p1	TAGCAGCCCGTAAAGGAAAC	AGACGAATCACACTTTTACCACT	50.0	39.1	57.9	58.0	201-204 bp	1.0	65.1
ycf12-trnG-UCC_p2	TCGTATCGGGACCACTTGTA	AGACGAATCACACTTTTACCACT	50.0	39.1	57.9	58.0	231-234 bp	1.0	63.6
ycf12-trnG-UCC_p3	GCGCTAATAGTCGTATCGGG	AGACGAATCACACTTTTACCACT	55.0	39.1	58.1	58.0	241-244 bp	1.0	63.4
ycf12-trnG-UCC_p4	CGGGACCACTTGTAATTGCT	AGACGAATCACACTTTTACCACT	50.0	39.1	58.2	58.0	225-228 bp	1.0	63.4
ycf12-trnG-UCC_p5	GGCGCTAATAGTCGTATCGG	AGACGAATCACACTTTTACCACT	55.0	39.1	58.1	58.0	242-245 bp	1.0	63.3