Hotspot

psbB-psbT

LSC · 73800-74004 · 205 bp

Back to Marshallia

Pi 0.0179

MarkerSeek score 58.80

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 205
variable_sites: 7
pi: 0.0179
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TTTTCGCTGGTATTGACCCA	TGAAATTTTCGGCGGTTCTC	45.0	45.0	57.4	57.0	377-409 bp	1.0	72.1
psbB-psbT_p2	TTTTCGCTGGTATTGACCCA	AGTTGAAATTTTCGGCGGTTC	45.0	42.9	57.4	58.0	380-412 bp	1.0	71.9
psbB-psbT_p3	TTTTCGCTGGTATTGACCCA	GTTGAAATTTTCGGCGGTTCT	45.0	42.9	57.4	58.0	379-411 bp	1.0	71.9
psbB-psbT_p4	TTTTCGCTGGTATTGACCCA	AGTTGAAATTTTCGGCGGTT	45.0	40.0	57.4	56.8	380-412 bp	1.0	70.6
psbB-psbT_p5	TTTTCGCTGGTATTGACCCA	GAAATTTTCGGCGGTTCTCT	45.0	45.0	57.4	56.7	376-408 bp	1.0	69.8