Hotspot

ndhC-trnV-UAC

LSC · 49928-51131 · 1204 bp

Back to Marshallia

Pi 0.00422

MarkerSeek score 63.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1204
variable_sites: 16
pi: 0.00422
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTCCTTTGCTAATCGGAGCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1338-1375 bp	1.0	77.6
ndhC-trnV-UAC_p2	CGGTCCTTTGCTAATCGGAG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	1340-1377 bp	1.0	76.7
ndhC-trnV-UAC_p3	CTCCGGTCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	1343-1380 bp	1.0	76.7
ndhC-trnV-UAC_p4	GTCCTTTGCTAATCGGAGCT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	1337-1374 bp	1.0	74.8
ndhC-trnV-UAC_p5	GGTCCTTTGCTAATCGGAGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.7	57.8	1339-1376 bp	1.0	74.5