Hotspot

psbC-trnS-UGA

LSC · 34805-35026 · 222 bp

Back to Marshallia

Pi 0.021

MarkerSeek score 59.10

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 222
variable_sites: 10
pi: 0.021
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	332-337 bp	1.0	81.4
psbC-trnS-UGA_p2	TCTTCGTAGGGCATTTGTGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.9	58.0	415-420 bp	1.0	81.3
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	GTCTTTCGGAGAGATGGCTG	55.0	55.0	58.2	58.1	340-345 bp	1.0	81.0
psbC-trnS-UGA_p4	TCTTCGTAGGGCATTTGTGG	GTCTTTCGGAGAGATGGCTG	50.0	55.0	57.9	58.1	423-428 bp	1.0	80.9
psbC-trnS-UGA_p5	TCTTCGTAGGGCATTTGTGG	TCTTTCGGAGAGATGGCTGA	50.0	50.0	57.9	58.1	422-427 bp	1.0	80.2