Hotspot

trnE-UUC-rpoB

LSC · 11943-12907 · 965 bp

Back to Marshallia

Pi 0.00078

MarkerSeek score 61.10

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 965
variable_sites: 3
pi: 0.00078
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GGGACTTGAACCCTCGTTTT	CTTCTGCCAAACCCTGATCA	50.0	50.0	58.0	57.8	1091-1166 bp	1.0	79.4
trnE-UUC-rpoB_p2	TAACCACTGGACCATAGGGG	CTTCTGCCAAACCCTGATCA	55.0	50.0	58.1	57.8	1050-1125 bp	1.0	78.7
trnE-UUC-rpoB_p3	CCCTCGTTTTCTCCGTGAAA	CTTCTGCCAAACCCTGATCA	50.0	50.0	58.1	57.8	1081-1156 bp	1.0	78.6
trnE-UUC-rpoB_p4	TTGAACCCTCGTTTTCTCCG	CTTCTGCCAAACCCTGATCA	50.0	50.0	58.1	57.8	1086-1161 bp	1.0	78.6
trnE-UUC-rpoB_p5	GGGACTTGAACCCTCGTTTT	TCTTCTGCCAAACCCTGATC	50.0	50.0	58.0	57.5	1092-1167 bp	1.0	77.1