Hotspot

trnC-GCA-petN

LSC · 9291-10122 · 832 bp

Back to Marshallia

Pi 0.00555

MarkerSeek score 59.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 10
pi: 0.00555
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TGTATATCGTTTTGGCGGCA	CAGCCCAAGCGAGACTTATT	45.0	50.0	58.0	58.0	626-956 bp	1.0	84.4
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCGAGACTTATT	50.0	50.0	58.4	58.0	589-919 bp	1.0	81.8
trnC-GCA-petN_p3	TGTATATCGTTTTGGCGGCA	ATTAAAGCAGCCCAAGCGAG	45.0	50.0	58.0	58.9	633-963 bp	1.0	77.5
trnC-GCA-petN_p4	TGTATATCGTTTTGGCGGCA	GACTACCATTAAAGCAGCCCA	45.0	47.6	58.0	58.0	640-970 bp	1.0	76.7
trnC-GCA-petN_p5	AGTTCAAATCTGGGTGCCG	CAGCCCAAGCGAGACTTATT	52.6	50.0	58.1	58.0	564-894 bp	1.0	76.3