Hotspot

rpl23

Genome · 80550-80825 · 276 bp

Back to Marchantia

Pi 0.0193

MarkerSeek score 32.90

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: Genome
length_bp: 276
variable_sites: 8
pi: 0.0193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	AAACCGCACTCAAAGGTAGG	TCACACAGATAAGCTCACGC	50.0	50.0	58.1	58.0	1106-1111 bp	1.0	82.4
rpl23_p2	AAACCGCACTCAAAGGTAGG	TGGCGAATTCACAGGTTCAA	50.0	45.0	58.1	58.0	910-915 bp	1.0	82.1
rpl23_p3	AAACCGCACTCAAAGGTAGG	TTGGCGAATTCACAGGTTCA	50.0	45.0	58.1	58.0	911-916 bp	1.0	82.1
rpl23_p4	CTCCTCGGTGTTGACTTGTT	TCACACAGATAAGCTCACGC	50.0	50.0	57.8	58.0	866-871 bp	1.0	81.7
rpl23_p5	TTCAAACCGCACTCAAAGGT	TCACACAGATAAGCTCACGC	45.0	50.0	58.2	58.0	1109-1114 bp	1.0	81.3