Hotspot

chlB

Genome · 24053-25594 · 1542 bp

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Pi 0.0129

MarkerSeek score 42.50

Primer available yes

Label chlB

Feature Metadata

label_name: chlB
feature_type: gene
region: Genome
length_bp: 1542
variable_sites: 30
pi: 0.0129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB_p1	GAGGTTCGAATCCTTCCGTC	CAAGTCGCACTTAAAAGCCG	55.0	50.0	58.1	58.0	2204-2235 bp	1.0	82.5
chlB_p2	AAATAGATATGGGGCGTCGC	CAAGTCGCACTTAAAAGCCG	50.0	50.0	57.9	58.0	2261-2292 bp	1.0	82.3
chlB_p3	GAGGTTCGAATCCTTCCGTC	AAGTCGCACTTAAAAGCCGA	55.0	45.0	58.1	58.1	2203-2234 bp	1.0	81.8
chlB_p4	TGGTCCTGTTATTCGGAGGT	CAAGTCGCACTTAAAAGCCG	50.0	50.0	58.1	58.0	2250 bp	0.5	59.2
chlB_p5	TGGTCCTGTTATTCGGAGGT	AAGTCGCACTTAAAAGCCGA	50.0	45.0	58.1	58.1	2249 bp	0.5	58.6