Hotspot

MapoCp088

Genome · 105329-108535 · 3207 bp

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Pi 0.0244

MarkerSeek score 57.30

Primer available yes

Label MapoCp088

Feature Metadata

label_name: MapoCp088
feature_type: gene
region: Genome
length_bp: 3207
variable_sites: 118
pi: 0.0244
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
MapoCp088_p1	TTCTCCATTGCTGAGGTGTT	TCCATTAGCTCAGTGGGGAA	45.0	50.0	57.0	58.0	1365 bp	0.5	52.1
MapoCp088_p2	TTCTCCATTGCTGAGGTGTT	TTCCATTAGCTCAGTGGGGA	45.0	50.0	57.0	58.0	1366 bp	0.5	52.1
MapoCp088_p3	TTTCTCCATTGCTGAGGTGT	TCCATTAGCTCAGTGGGGAA	45.0	50.0	57.0	58.0	1366 bp	0.5	52.1
MapoCp088_p4	TTTCTCCATTGCTGAGGTGT	TTCCATTAGCTCAGTGGGGA	45.0	50.0	57.0	58.0	1367 bp	0.5	52.1
MapoCp088_p5	TTCTCCATTGCTGAGGTGTT	CTCAGTGGGGAAGAAGTTGG	45.0	55.0	57.0	57.8	1357 bp	0.5	50.9