Hotspot

MapoCp023

Genome · 29909-36319 · 6411 bp

Back to Marchantia

Pi 0.0238

MarkerSeek score 55.30

Primer available yes

Label MapoCp023

Feature Metadata

label_name: MapoCp023
feature_type: gene
region: Genome
length_bp: 6411
variable_sites: 231
pi: 0.0238
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
MapoCp023_p1	TGAATTTGTTACTTGGCAATCA	AAAAAGTATCCAAAACACTGTT	31.8	27.3	54.8	52.7	1235 bp	0.5	20.0
MapoCp023_p2	TGAATTTGTTACTTGGCAATCA	AAAAGTATCCAAAACACTGTTT	31.8	27.3	54.8	52.7	1234 bp	0.5	20.0
MapoCp023_p3	TGAATTTGTTACTTGGCAATCA	AAAGTATCCAAAACACTGTTTT	31.8	27.3	54.8	52.7	1233 bp	0.5	20.0
MapoCp023_p4	TGAATTTGTTACTTGGCAATCA	AAGTATCCAAAACACTGTTTTT	31.8	27.3	54.8	52.7	1232 bp	0.5	20.0
MapoCp023_p5	TGAATTTGTTACTTGGCAATCA	AAAGTATCCAAAACACTGTTTTT	31.8	26.1	54.8	53.5	1233 bp	0.5	20.0