Hotspot

cysT-MapoCt128

Genome · 95050-95144 · 95 bp

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Pi 0.0386

MarkerSeek score 68.00

Primer available yes

Label cysT-MapoCt128

Feature Metadata

label_name: cysT-MapoCt128
feature_type: igs
region: Genome
length_bp: 95
variable_sites: 7
pi: 0.0386
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cysT-MapoCt128_p1	GGCCTAATTCTTGCTTGGGT	GATTCGAACCGAGAAGCCTT	50.0	50.0	58.1	58.0	883-889 bp	1.0	83.2
cysT-MapoCt128_p2	TGGCCTAATTCTTGCTTGGG	GATTCGAACCGAGAAGCCTT	50.0	50.0	58.1	58.0	884-890 bp	1.0	83.1
cysT-MapoCt128_p3	TGCATGGTGTTTAGGTGCAT	GATTCGAACCGAGAAGCCTT	45.0	50.0	58.1	58.0	602 bp	0.5	60.1
cysT-MapoCt128_p4	TGCATGGTGTTTAGGTGCAT	AAGTCGTGGGTTCAAATCCC	45.0	50.0	58.1	58.1	435 bp	0.5	59.7
cysT-MapoCt128_p5	TGCATGGTGTTTAGGTGCAT	GGTGATGAAAGTCGTGGGTT	45.0	50.0	58.1	58.1	443 bp	0.5	59.6