Hotspot

petD-rpoA

Genome · 73691-73801 · 111 bp

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Pi 0.0601

MarkerSeek score 67.50

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: Genome
length_bp: 111
variable_sites: 8
pi: 0.0601
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TGGTTAGGAATTGGAGCTGC	CAGTAGAACAAGTTTTGGAAGCA	50.0	39.1	57.9	57.5	245-252 bp	1.0	57.6
petD-rpoA_p2	TGGTTAGGAATTGGAGCTGC	TCAGTAGAACAAGTTTTGGAAGC	50.0	39.1	57.9	57.2	246-253 bp	1.0	55.5
petD-rpoA_p3	GGTTAGGAATTGGAGCTGCT	CAGTAGAACAAGTTTTGGAAGCA	50.0	39.1	57.6	57.5	244-251 bp	1.0	55.2
petD-rpoA_p4	TGGTTAGGAATTGGAGCTGC	AGTAGAACAAGTTTTGGAAGCA	50.0	36.4	57.9	56.1	244-251 bp	1.0	54.6
petD-rpoA_p5	GGTTAGGAATTGGAGCTGCT	TCAGTAGAACAAGTTTTGGAAGC	50.0	39.1	57.6	57.2	245-252 bp	1.0	53.2