Hotspot

MapoCp047-MapoCp048

Genome · 63685-64151 · 467 bp

Back to Marchantia

Pi 0.0439

MarkerSeek score 64.80

Primer available yes

Label MapoCp047-MapoCp048

Feature Metadata

label_name: MapoCp047-MapoCp048
feature_type: igs
region: Genome
length_bp: 467
variable_sites: 31
pi: 0.0439
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
MapoCp047-MapoCp048_p1	GCTCTCCCGTATTTCCAGAC	GGATGTAGCGCAGTTTGGTA	55.0	50.0	57.8	58.0	1246-1256 bp	1.0	83.2
MapoCp047-MapoCp048_p2	GCTCTCCCGTATTTCCAGAC	GGTTCGAATCCTGTCATCCC	55.0	55.0	57.8	58.0	1196-1206 bp	1.0	83.2
MapoCp047-MapoCp048_p3	GCTCTCCCGTATTTCCAGAC	TCGGTAGAACGTAGGTCTCC	55.0	55.0	57.8	58.0	1078-1080 bp	1.0	82.8
MapoCp047-MapoCp048_p4	ATCATAAGCCAACCCTGTGC	GGATGTAGCGCAGTTTGGTA	50.0	50.0	58.2	58.0	1359-1369 bp	1.0	82.7
MapoCp047-MapoCp048_p5	ATCATAAGCCAACCCTGTGC	TCGGTAGAACGTAGGTCTCC	50.0	55.0	58.2	58.0	1191-1193 bp	1.0	82.3