Hotspot

MapoCt119-accD

Genome · 57951-58064 · 114 bp

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Pi 0.0512

MarkerSeek score 64.00

Primer available yes

Label MapoCt119-accD

Feature Metadata

label_name: MapoCt119-accD
feature_type: igs
region: Genome
length_bp: 114
variable_sites: 9
pi: 0.0512
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
MapoCt119-accD_p1	AAGTCAAGGGTTCGAATCCC	AAGCGCCAATTAATCCACCA	50.0	45.0	57.5	58.2	202-272 bp	1.0	78.1
MapoCt119-accD_p2	AAGTCAAGGGTTCGAATCCC	AGCGCCAATTAATCCACCAA	50.0	45.0	57.5	58.2	201-271 bp	1.0	78.1
MapoCt119-accD_p3	AAGTCAAGGGTTCGAATCCC	GCGCCAATTAATCCACCAAA	50.0	45.0	57.5	57.3	200-270 bp	1.0	74.0
MapoCt119-accD_p4	TGAAGTCAAGGGTTCGAATCC	AGCGCCAATTAATCCACCAA	47.6	45.0	57.9	58.2	203-273 bp	1.0	73.6
MapoCt119-accD_p5	TGAAGTCAAGGGTTCGAATCC	AAGCGCCAATTAATCCACCA	47.6	45.0	57.9	58.2	204-274 bp	1.0	73.5