Hotspot

chlB-MapoCp020

Genome · 25595-25768 · 174 bp

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Pi 0.0594

MarkerSeek score 63.60

Primer available yes

Label chlB-MapoCp020

Feature Metadata

label_name: chlB-MapoCp020
feature_type: igs
region: Genome
length_bp: 174
variable_sites: 15
pi: 0.0594
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB-MapoCp020_p1	GCAAGCTGCTTGGTTTTCAA	CAAGTCGCACTTAAAAGCCG	45.0	50.0	58.1	58.0	1140-1168 bp	1.0	84.0
chlB-MapoCp020_p2	GCAAGCTGCTTGGTTTTCAA	AAGTCGCACTTAAAAGCCGA	45.0	45.0	58.1	58.1	1139-1167 bp	1.0	83.4
chlB-MapoCp020_p3	TACAGAAGTCGGGGACATGA	CAAGTCGCACTTAAAAGCCG	50.0	50.0	57.8	58.0	946 bp	0.5	57.9
chlB-MapoCp020_p4	TCATACAGAAGTCGGGGACA	CAAGTCGCACTTAAAAGCCG	50.0	50.0	57.8	58.0	949 bp	0.5	57.9
chlB-MapoCp020_p5	TACAGAAGTCGGGGACATGA	AAGTCGCACTTAAAAGCCGA	50.0	45.0	57.8	58.1	945 bp	0.5	57.3