Hotspot

atpI-atpH

Genome · 17637-18013 · 377 bp

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Pi 0.0332

MarkerSeek score 64.60

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: Genome
length_bp: 377
variable_sites: 19
pi: 0.0332
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GCGAATCTATGGAAGGGCAT	GCAGCAATAACAGAAGCAGC	50.0	50.0	57.8	58.1	433-441 bp	1.0	82.7
atpI-atpH_p2	GCGAATCTATGGAAGGGCAT	AAGCAGCAGAAATCAAGGGG	50.0	50.0	57.8	58.4	420-428 bp	1.0	80.0
atpI-atpH_p3	GCGAATCTATGGAAGGGCAT	CCAGCAGCAATAACAGAAGC	50.0	50.0	57.8	57.4	436-444 bp	1.0	78.9
atpI-atpH_p4	GCGAATCTATGGAAGGGCAT	GCAGCAGAAATCAAGGGGTT	50.0	50.0	57.8	58.7	418-426 bp	1.0	77.7
atpI-atpH_p5	GCGAATCTATGGAAGGGCAT	GAAGCAGCAGAAATCAAGGG	50.0	50.0	57.8	57.1	421-429 bp	1.0	76.1