Hotspot

rps7-ndhB

Genome · 1360-1513 · 154 bp

Back to Marchantia

Pi 0.0465

MarkerSeek score 67.80

Primer available yes

Label rps7-ndhB

Feature Metadata

label_name: rps7-ndhB
feature_type: igs
region: Genome
length_bp: 154
variable_sites: 11
pi: 0.0465
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-ndhB_p1	ACTCATAAAATGGCAGAAGCT	ACATTCTGGTAAAATAGTACTTCCA	38.1	32.0	55.5	56.0	245-259 bp	1.0	47.5
rps7-ndhB_p2	AACTCATAAAATGGCAGAAGCT	ACATTCTGGTAAAATAGTACTTCCA	36.4	32.0	56.3	56.0	246-260 bp	1.0	47.5
rps7-ndhB_p3	AAACTCATAAAATGGCAGAAGCT	ACATTCTGGTAAAATAGTACTTCCA	34.8	32.0	57.0	56.0	247-261 bp	1.0	47.5
rps7-ndhB_p4	ACTCATAAAATGGCAGAAGCT	ACATTCTGGTAAAATAGTACTTCCAT	38.1	30.8	55.5	56.4	245-259 bp	1.0	47.5
rps7-ndhB_p5	GAAACTCATAAAATGGCAGAAGCT	ACATTCTGGTAAAATAGTACTTCCA	37.5	32.0	58.0	56.0	248-262 bp	1.0	47.5